“Men love to wonder, and that is the seed of science.” — Ralph Waldo Emerson

Hollie Siebler

Instructor
Cell & Molecular Biology, Cancer Biology

Ph.D. – University of Nebraska Medical Center, 2015
B.A. – Dana College, 2010

Department of Biology
Creighton University
2500 California Plaza
Omaha, NE 68178-0103
Office: Eppley 437
Phone: 402-280-3876
HollieSiebler@creighton.edu

Courses Offered

  • BIO 149 – Human Biology for Nonmajors
  • BIO 159 – Human Biology for Nonmajors (with lab)
  • BIO 202 – General Biology: Cellular and Molecular
  • BIO 205 – General Biology: Organismal and Population Laboratory
  • BIO 206 – General Biology: Cellular and Molecular Laboratory
  • BIO 362 – Cell Structure and Function
  • BIO 532 – Current Topics in Molecular Biology (Cancer Biology)

Research Interests

My research was focused on DNA Polymerase (Pol) Zeta. This is an error-prone DNA polymerase. It specializes in inserting nucleotides across from bulky lesions in template DNA (for example, from harmful UV exposure). This allows cells that would normally die from the damage to survive and replicate. The cost is that Pol Zeta often makes mutations as it bypasses damage. It is known that Pol Zeta is a major contributor to formation of cancer mutations in result to environmental damage. I was studying the recruitment mechanism of this enigmatic polymerase because it is both friend and foe. I also worked in a breast cancer lab studying the protein TSG101 for one year. I do not have a current active lab at Creighton unfortunately.

Scholarly Works

Publications

Baranovskiy, A. G., H. M. Siebler, Y. I. Pavlov, and T. H. Tahirov. 2018. Iron–Sulfur Clusters in DNA Polymerases and Primases of Eukaryotes. Methods in Enzymology 599:1-20. DOI:10.1016/bs. mie.2017.09.003

Stepchenkova, E. I., E. R. Tarakhovskaya, H. M. Siebler, and Y. I. Pavlov. 2017. Defect of Fe-S cluster binding by DNA polymerase δ in yeast suppresses UV-induced mutagenesis, but enhances DNA polymerase ζ – dependent spontaneous mutagenesis. DNA Repair 49:60-69. DOI:10.1016/j .dnarep.2016.11.004

Siebler, H. M., A. G. Lada, A. G. Baranovskiy, T. H. Tahirov, and Y. I. Pavlov. 2014. A novel variant of DNA polymerase ζ, Rev3δC, highlights differential regulation of Pol32 as a subunit of polymerase δ versus ζ in Saccharomyces cerevisiae. DNA Repair 24:138-149. DOI:10.1016/j .dnarep.2014.04.013

Hlady, R. A., S. Novakova, J. Opavska, D. Klinkebiel, S. L. Peters, J. Bies, J. Hannah, J. Iqbal, K. M. Anderson, H. M. Siebler, L. M. Smith, T. C. Greiner, D. Bastola, S. Joshi, O. Lockridge, M. A. Simpson, D. W. Felsher, K.-U. Wagner, W. C. Chan, J. K. Christman, and R. Opavsky. 2012. Loss of Dnmt3b function upregulates the tumor modifier Ment and accelerates mouse lymphomagenesis. Journal of Clinical Investigation 122 1:163-177. DOI:10.1172/JCI57292< /p>

Baranovskiy, A. G., A. G. Lada, H. M. Siebler, Y. Zhang, Y. I. Pavlov, and T. H. Tahirov. 2012. DNA polymerase δ and ζ switch by sharing accessory subunits of DNA polymerase δ. Journal of Biological Chemistry 287 21:17281-17287. DOI:10.1074/jbc. M112.351122

Lee, H.-T., C. Carr, H. Siebler, L. Waters, I. Khutsishvili, F. Iseka, B. Domack, C. M. Olsen, and L. A. Marky. 2011. A thermodynamic approach for the targeting of nucleic acid structures using their complementary single strands. Methods in Enzymology 492:1-26. DOI:10. 1016/B978-0-12-381268-1.00013-6